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Effects of different concentrations of guanidine hydrochloride(50-01-1) solution on the fluorescence changes of different oligopeptide carbonyl derivatives

The results of the determination of N-terminal α-carbonyl and quinoxaline derivatives and fluorescence in potassium iodide, ethylene glycol, guanidine hydrochloride(50-01-1) and sodium chloride solution show that the carbonyl fluorescence of different primary structure oligopeptides. The fluorescence changes of the oligonucleotides were different in the different concentrations of ethylene glycol and guanidine hydrochloride solution. 

The results showed that the emission peak of cyanamide oligonucleotides was shifted by blue, and the positions of the glycyrrhizin derivatives were different from those of the other groups. Diol or guanidine hydrochloride(50-01-1) solution, but this difference gradually decreases with the extension of the peptide chain. The above results suggest that although oligosaccharides (containing 2-6 amino acid residues) are difficult to form in the solution secondary structures, but their spatial conformation may not be random; the longer the oligopeptide chain, the structure is relatively stable.

 

 

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