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Analysis of Factors Affecting the Detection of Protein Tyrosine Phosphatase-2 Autoantibody Radical Ligand by TRIS(77-86-1) Buffer Solution

Methods: 186 batches of IA-2A were detected by radioligand method, and the results were retrospectively analyzed and summarized. The sensitivity and specificity of the method were verified by the standardized examination (DASP 2005). (CV) was 3.1% ~ 9.5%, and the inter-batch CV was 5.5% ~ 11.7%. With the signal / noise ratio (S / N) ≥50 as the ideal standard for detecting IA-2A, 186 batch (86.6%), failed 25 batches (13.4%). The failure rate (60% of the total failed batch) in the high temperature operating environment [(32.7 ± 6.3) ° C) was significantly higher than that of the low temperature operating environment [(11.8 ± 5.7 ° C, 16% of the total batch) The high temperature operating environment S / N (55.1 ± 6.9) was significantly lower than the low temperature operating environment (68.5 ± 6.2, P <0.01). In the failed batches, 68% (17/25) were caused by TRIS(77-86-1) buffered saline, and other reasons were marked failure. DASP 2005 feedback results showed that IA-2A detection sensitivity of 72%, 99% specificity, the receiver operating characteristics (ROC) curve analysis showed that the area under the curve 0.825 ± 0.048 (0.730 ~ 0.919).

Conclusion: The influencing factors of IA-2A detection by radiation ligand method, especially TRIS(77-86-1) buffer salt solution, should be brought to the attention of the operator and controlled to ensure the results are stable and reliable.

The influencing factors of protein tyrosine phosphatase-2 autoantibody (IA-2A) radioligand assay were analyzed.



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