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The extraction of recombinant porcine growth hormone by guanidine hydrochloride(cas 50-01-1)

The recombinant plasmid pgh / E.coli BL21 was treated with enzymatic hydrolysis, ultrasonic treatment, ultrasonic treatment and freeze-thawing of liquid nitrogen. The recombinant porcine growth hormone (rpGH) was extracted with guanidine hydrochloride(cas 50-01-1).
The results showed that the extraction rate of rpGH was 72.16%, and the inclusion of rpGH in Escherichia coli was studied by urea method. The results showed that the extraction rate of rpGH was up to 72.16%. The rate of rpGH extraction was only 49.82%.

 

 

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