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Thiourea/ urea/ guanidine hydrochloride(50-01-1) method

Establishment of normal human sperm head protein by bi - directional protein electrophoresis(2DE) technique using human sperm proteome analysis. 

Methods: Protein separation was performed on whole sperm and sperm head protein by solid phase pH gradient SDS electrophoresis (IPG DALT). The difference of protein spots between sperm protein and sperm protein was compared with image analysis software The Among them, the whole sperm protein extraction compared thiourea /urea /guanidine hydrochloride and Kit /guanidine hydrochloride(50-01-1) two extraction methods. 

Results: The total spermatozoa 2DE image protein spots of thiourea / urea /guanidine hydrochloride and Kit /guanidine hydrochloride(50-01-1) were 802 and 797, respectively, of which 4 92 were the same, and the images obtained by the two methods were integrated And the whole sperm protein image has 1107 protein spots; sperm head image protein spots have 428, matched, all derived from the whole sperm protein image.

Conclusion: The protein profile of sperm head was established by two kinds of protein extraction methods.



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