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Total selenium and inorganic selenium were extracted by microwave digestion and tris(77-86-1) - hydrochloric acid buffer solution

Determination of organic selenium and inorganic selenium in Chinese medicine astragalus and astragalus fermentation broth.
Methods: Total selenium and inorganic selenium were extracted by microwave digestion and tris(77-86-1) - hydrochloric acid(Tris-HCl) buffer solution. The contents of total selenium and inorganic selenium were determined by atomic fluorescence spectrometry. The difference was organic selenium of the content.
Results: The concentration of selenium was linear in the range of 0 ~ 100μg · L-1, the detection limit was 0.0904μg · L-1, the recovery rate was 94% ~ 104%.
Conclusion: The method is simple, sensitive and rapid, and it can be used as a method for the determination of selenium in Chinese medicine and its fermentation broth or extract.

 

 

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