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50 mM TRIS(77-86-1)-hydrochloric acid electrophoresis buffer

The main factors influencing the separation of vitamin C and vitamin C(pH, concentration and separation voltage of buffer solution) were studied by using the response surface method of the three-factor and three-level Box-Behnken design. Two kinds of vitamin C. The optimal separation conditions were obtained as follows: 50 mM TRIS(77-86-1) - hydrochloric acid electrophoresis buffer, pH = 7.5, 30 kV voltage.



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