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The ordered structure of adsorbed BSA adsorbed on PEG-600 was increased with the increase of guanidine hydrochloride(cas 50-01-1) concentration

The results of FTIR and DSC showed that the ordered structure of adsorbed BSA adsorbed on PEG-600 was increased with the increase of guanidine hydrochloride(cas 50-01-1) concentration or ammonium sulfate concentration. In the fixed system (fixed guanidine hydrochloride and ammonium sulfate concentration), the FTIR results show that the ordered secondary structure of adsorbed BSA decreases with the increase of surface coverage. The stability increases gradually, indicating that the total ordered structure of adsorbed BSA is still increasing. The adsorption enthalpy change ΔH and the calculated entropy change ΔS and Gibbs free energy variable ΔG were all negative values, which indicated that the adsorption of denatured BSA on PEG-600 surface was spontaneous and enthalpy-driven. The analysis of the thermodynamic function shows that the effect of guanidine hydrochloride on the four secondary processes of adsorption is different. The addition of guanidine hydrochloride(cas 50-01-1) reduces the adsorption affinity between the BSA molecule and the hydrophobic surface (secondary process (a)) and dehydration (Secondary process (c)) facilitates the folding of the denatured BSA on the surface of the hydrophobic adsorbent (secondary process (b)) and the dehydration (secondary process (d)) within the BSA molecule. The results show that the thermodynamic functions ΔH, ΔS and ΔG and the net adsorption components ΔHA, ΔSA and ΔGA are not monotonically changed, and their absolute values are increased first and then decreased. When the concentration of guanidine hydrochloride is 0.6 mol·L- 1 has the maximum value. While the addition of ammonium sulfate on the four sub-processes have a great role in promoting. With the increase of the concentration of ammonium sulfate in the system, the absolute value of the thermodynamic function and its net adsorption component and net desorption component are monotonically increasing.

 

 

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