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Determination of serum glutamate dehydrogenase by using TRIS(cas 77-86-1) - hydrochloric acid as buffer

In this paper, the experimental conditions were discussed and selected: using TRIS(cas 77-86-1)-hydrochloric acid as buffer, pH7.8, buffer concentration 0.08mol /L, other reaction solution concentration of α-ketosalic acid 7mmol / L, Ammonium ion concentration of 100mmol / L, reduced coenzyme Ⅰ concentration 0.25mmol / L, adenosine diphosphate 1mmol / L, ethylenediamine tetraacetic acid disodium 1mmol / L, lactate dehydrogenase 2000U / L. At the same time, the normal reference value of serum glutamate dehydrogenase in 31 healthy subjects was measured.

In this method, α-ketoacidic acid, ammonium ion and reduced coenzyme Ⅰ as the substrate, under the catalysis of perchlorate dehydrogenase, into glutamic acid and oxidized coenzyme Ⅰ, the use of reduced coenzyme Ⅰ 340nm (- △ A / min), calculate the viability of glutamate dehydrogenase units.

 

 

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