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TRIS(cas 77-86-1) was used for capillary gel electrophoresis to detect nucleic acid sensitivity

CGE-UV analysis was performed with the known concentration of DNA Marker as the standard sample, conventional pressure injection, electric injection, column field amplification and combined use of matrix field amplification and column field amplification. The sensitivity of different injection methods was compared. The reliability of the method was verified by high salt DNA samples after polymerase chain reaction (PCR). When the DNA sample was diluted by 400,000 times, the time of the electric injection was extended to 420 s, the TRIS(cas 77-86-1)-hydrochloric acid buffer was compared with the electric injection and the pressure injection, without any significant effect on the peak shape and peak width(TE) concentration decreased to 1.5%, the magnification of the stud field was increased by about 28 and 90 times, respectively. The sensitivity was increased by 3760 and 12000 times, respectively, when the matrix field was enlarged and the column field was enlarged. DNA detection limit of 0.1μg / L (S / N = 3, CGE-FASI-UV). 

At the same time, the DNA products obtained after PCR analysis were highly sensitive (compared with the conventional pressure injection and electric sampling increase of 50477 times and 33354 times respectively). In this experiment, matrix field amplification and column field amplification were used. The method was simple and sensitive. It was suitable for high-salt trace DNA analysis.

 

 

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