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The TRIS(cas 77-86-1) buffer solution was used to determine the quercetin content in the ethanol extract of Podocarpus

Objective: To determine the content of quercetin in ethanol extract of Mongolian medicine by high performance capillary electrophoresis.

Methods: 10 mmol·L-1 borax-30 mmol·L-1 TRIS(cas 77-86-1) buffer solution (pH 9.5) was used as electrophoresis buffer, and subjected to electrophoretic separation at 20 ℃ and 20kV constant pressure, and detected at a wavelength of 254 nm.

The average recoveries of quercetin were 95.64% and the RSD was 3.5% (n = 6). The results showed that the concentration of quercetin was 95.64% and the RSD was 3.5% (n = 6).

Conclusion: The method is simple, accurate and rapid.

 

 

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