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Experimental study on preparation of acellular dermal matrix by sodium dodecyl sulfate(cas 151-21-3)

The optimal concentration of decalative dermal matrix prepared by sodium dodecyl sulphate(cas 151-21-3) is 0.25% and the best time is 40 h. Compared with other methods, it is simple, rapid, effective, inexpensive and popularizable. Clinical emergency repair for valuable time.

The optimum concentration and time of the preparation of decellular dermal matrix were studied.

Methods: The patients were divided into 6 groups randomly divided into 6 groups, each about 1 cm × 1 cm, were placed in 0.1%, 0.25%, 0.5%, 1.0%, 2.5%, 5.0%, Dissolved cells in sodium alkyl sulfate solution were extracted once every 8 h, and 15 specimens were drawn from each other, and the specimens were normal and histologically observed.

Results: After observing the effect of sodium dodecyl sulfate(cas 151-21-3) for 8 h, 0.25% group could completely separate the epidermis from dermis. The results of light microscopy showed that 0.25% sodium dodecyl sulfate could be used after 40 h Of the cells and cell debris off the net, the formation of acellular dermal matrix. Preparation of acellular dermal matrix, mainly by the collagen fiber network structure, the other group of 120 h can not form a real acellular dermal matrix.

 

 

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