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MES(cas 4432-31-9), Free Acid

You might have been used MES(cas 4432-31-9) as buffer in electrophoresis or culture media. You may be curious about more information.

MES, selected and described by Good et al, is a buffering agent used in biochemistry and molecular biology. It is a zwitterionic, morpholinic buffer used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography, while is only useful for a pH range of 5.5 – 6.7. 

MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. MES is regarded as an excellent buffer for use in capillary electrochromatography due to its low ionic mobility. MES(cas 4432-31-9) is often employed as a substitute for the highly toxic cacodylate, as well as a substitute for the non-zwitterionic buffers citrate and malate. 

The MES free acid or MES free acid monohydrate can be mixed with the MES sodium salt to obtain the desired pH. Alternatively, MES free acid or MES(cas 4432-31-9) free acid monohydrate can be titrated with sodium hydroxide to achieve the desired pH.



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