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MES(cas 4432-31-9) and 1M Tris-HCl

MES(cas 4432-31-9):
Alias: 2-Morpholinoethanesulfonic acid
CAS Number: 4432-31-9
Molecular formula: C6H13NO4S
Molecular weight: 195.237
H bond acceptor: 5
H key supply: 5
Molecular weight: 195.238
Twist key: 2
Hydrophobic parameters: -3.07
Number of violations: 0
Appearance: white crystalline powder
1M Tris-HCl:
Component Concentration: 1 M Tris-HCl (pH 7.4, 7.6, 8.0)
Formulation: 1L
Configuration method: 1. Weigh 121.1gTris placed in a 1L beaker. 
2. Add about 800 mL of deionized water and stir well to dissolve. 
3. Add the desired pH by adding concentrated hydrochloric acid. 
PH concentration of HCl
7.4 about 70mL
7.6 about 60mL
8.0 about 42mL
4. Dissolve the solution to 1L. 
5. High temperature and high pressure sterilization, room temperature preservation. 
Note: The solution should be cooled to room temperature and then set the pH value, because the pH value of the Tris solution varies greatly with temperature, and the pH of the solution is reduced by about 0.03 units for every 1 °C increase in temperature.

 

 

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