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HEPES(cas 7365-45-9) buffer is necessary for the cell culture process

In the cell culture process, HEPES(cas 7365-45-9) buffer is necessary. So what is the preparation and use of HEPES buffer? HEPES buffer is a weak, in the open culture conditions, if the addition of HEPES, to prevent the pH increase in the culture medium, which will maintain the pH of 7.0 or so. The HEPES molecular weight is 238.31, chemical name: 4-hydroxyethyl piperazineethanesulfonic acid, the formula: C8H18N2O4S. HEPES(cas 7365-45-9) is commonly used in biological buffers, pH buffer range: 6.8-8.2, used in biochemical diagnostic kits, DNA /RNA extraction kits and PCR diagnostic kits.

 

 

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