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RNA extraction solution: HEPES(cas 7365-45-9) and sodium chloride

The invention first provides an enteric virus RNA extraction kit comprising the following components: (1) RNA extraction solution I: sodium dodecyl sulfate, tramadol, guanidine isothiocyanate and 100-400 g /ml magnetic beads; (2) RNA extraction solution II: containing HEPES(cas 7365-45-9) and sodium chloride; (3) RNA extraction solution III: containing tramadol and sodium chloride; and (4) RNA extraction solution IV: silicone oil.

The extraction kit provided by the invention can cooperate with the automated instrument to carry out the quantitative detection of enterovirus RNA; the kit has high RNA extraction rate and high detection sensitivity; the RNA concentration in each sample can be measured quickly and accurately diagnosis of various types of pathogens to provide a reliable experimental basis. The present invention also provides a magnetic bead RNA extraction kit comprising an RNA eluate and a kit for extracting RNA in one step. The three kinds of kits provided by the invention can effectively extract different types of intestinal viral RNA of anal swabs, feces and throat swabs containing various inhibitors.

 

 

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