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Do experiments with HEPES(cas 7365-45-9)

N, N'-ethylene-bis [2- (2-hydroxyphenyl) glycoside)] were prepared by UV absorption spectroscopy and fluorescence spectroscopy at pH 7.4, 0.1 mol * L-1 HEPES(cas 7365-45-9) and room temperature Amino acid(EHPG) titration.

The results show that aluminum (Ⅲ) forms a 1: 1 complex with EHPG, and the UV absorption spectra of aluminum (Ⅲ) and EHPG show an absorption peak at 235 and 291 nm. The molar absorptivity of the complex at 235 nm is 1.27 × 104 cm-1 * mol-1 * L; the conditional stability constant is lg K = 14.20 ± 0.03; the fluorescence quenching of EHPG by aluminum (Ⅲ) is due to the dissociation of phenolic hydroxyl protons of EHPG and the coordination with aluminum (Ⅲ).



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