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HEPES(7365-45-9) medium for the regulation of T cells cultured and induced differentiation

The present invention provides an in vitro expansion medium for adult regulatory T cells and a method of using the same, which comprises transforming growth factor-beta (1-4 ng / mL), bone morphogenetic protein 4 (8-12 ng / mL), Recombinant human interleukin-2 (300-500 U / mL), rapamycin (80-100 nM / mL), all-trans retinoic acid (1-4 uM / mL), HEPES(7365-45-9) (20-30 mM / mL), L-glutamine (2 mM / mL), 2-mercaptoethanol (40-50 uM / mL), 5% human AB serum, CD3CD28 beads, penicillin (50 U / mL) Streptomycin (50ug / mL). The use of the culture medium to regulate the proliferation of T cells and induced differentiation, not only can shorten the regulatory T cell proliferation, differentiation time, but also access to high purity Foxp3CD4 + CD25 + CD127-regulatory T cells. Therefore, the regulatory T cells can be applied to clinical anti-transplant immune rejection, autoimmune diseases allergic disease treatment, etc., to carry out a new type of clinical cell therapy.



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