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Red blood cells are suspended in HEPES(7365-45-9) and plasma

In this paper, the research work is divided into two parts. In the biochemical level, the effect of 5-HT on erythrocytes after carbonyl stress is explored. (1) Two kinds of red blood cell suspensions with different concentrations of 5-HT on MDA HEPES(7365-45-9) and plasma apparent viscosity. The results showed that MDA could increase the apparent viscosity of erythrocyte suspension significantly in both systems. When MDA was induced by erythrocyte carbonyl stress, 5-HT was added, and the apparent viscosity of erythrocyte was further increased. HT can not repair damage to red blood cells caused by MDA. (2) Determination of different concentrations of 5-HT on MDA caused by erythrocyte carbonyl stress after the osmotic fragility changes. The results showed that the erythrocyte osmotic fragility increased significantly after MDA induced erythrocyte carbonyl stress, which indicated that MDA could cause damage to red blood cells. And different concentrations of 5-HT can not inhibit this damage. (3) Under the fluorescence microscope to observe the different treatment group of red blood cell morphology, found in both systems MDA can cause erythrocyte aggregation and deformation, continue to join 5-HT will exacerbate this degree of change, and in the plasma suspension, despite the high Concentration of 5-HT (50 × 10 ~ (-8) mol /L) treatment can still find the shape of the complete red blood cells, suggesting that there may be some plasma to protect the integrity of red blood cell morphology. 

Second, at the level of the whole animal to explore the ability of lysine to remove MDA will be treadmill exercise adaptive training rats were randomly divided into four groups, a quiet control group and three exercise exhaust group, three exercise exhaust group Respectively, the normal saline group, lysine group and MDA group; quiet control group were given the same dose of saline. Exercise intensity The exercise load model established by Bedford, immediately after the death of all rats, eye blood to take blood to prepare serum. The concentrations of MDA, lactic acid and lysine in each treatment group were measured and analyzed. The results showed that the concentration of lactic acid in the serum after exhaustive exercise increased, which was consistent with the theory of lactic acid accumulation in exercise physiology. A large amount of MDA was produced in the serum of exhaustive exercise, and lysine was given to reduce the amount of MDA produced.



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