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Sensitivity of Polymers to Fe ~ (3+) and Inorganic Phosphate (Pi) in HEPES(7365-45-9) Buffer Solution(pH 7.0)

In this paper, the following four aspects of the work carried out: 

1. In boron trifluoride ether (BFEE) by electrochemical polymerization of commercially available 9-amino fluorene monomer obtained water-soluble poly (9-amino fluorene (3) and inorganic phosphate (Pi) in HEPES(7365-45-9) buffer solution (pH 7.0) were studied. When Fe ~ (3+) was added, the P9AF fluorescence was almost quenched, and the fluorescence of P9AF-Fe ~ (3+) quenching system was recovered by Pi. The mechanism of P9AF was successfully studied by UV absorption and transmission electron microscopy (TEM). The detection of Fe ~ (3+) in the Ganjiang River water samples and the detection of Fe ~ (3+) and Pi in living cells were successfully applied.

2. Acetate (AcO ~ -) was effectively detected by fluorescence quenching in HEPES(7365-45-9) buffer (pH 7.4) using water-soluble P9AF synthesized by BFEE. The addition of AcO ~ - quenched P9AF80% fluorescence, while other common anions did not find this phenomenon. At the same time, the ability of P9AF to recognize some other carboxylic acids was studied. It was found that the number of carboxyl groups and the substituents affected the interaction between P9AF and carboxylic acid (salt). It is confirmed by hydrogen absorption analysis and quantitative calculation that hydrogen bond is the main mode of action between P9AF and carboxylic acid (salt). The Fe (3+) was effectively detected in ethanol system by using poly (9-fluorenecarboxylic acid) (PFCA) synthesized by BFEE as a highly selective fluorescence sensor. Fe ~ (3+) almost completely quenches the fluorescence of PFCA, while the addition of other common metal cations, anions, 20 kinds of natural amino acids, organic acids and sugars did not change significantly. 

3. The interaction between PFCA and Fe ~ (3+) enhances the aggregation of the polymer chains, which causes the quenching of PFCA fluorescence. Ultraviolet absorption spectroscopy and transmission electron microscopy were preliminarily demonstrated by this mechanism. 

4. The sensing properties of Pd-2+ in ethanol system were investigated by using polyacrylone (PBA), a polycyclic aromatic polymer, in the mixed system of BFEE and acetonitrile. Pd ~ (2+) can quench most of the fluorescence of PBA, while the addition of fluorescence intensity of other ions did not change significantly. The quenching mechanism may be due to the fact that Pd ~ (2+) interacts with two or more links in the PBA to enhance the aggregation of the polymer chains, which causes strong quenching of the PBA fluorescence. UV absorption spectroscopy and transmission electron microscopy confirmed the mechanism. This PBA-based fluorescence sensor was successfully used to detect Pd ~ (2+) in crop and environmental samples.



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