Dopamine is dissolved in the TRIS(77-86-1) buffer solution
    Experimental Study on Volatilization of TRIS(77-86-1) in the Process of Energy Storage
    Analysis of Factors Affecting the Detection of Protein Tyrosine Phosphatase-2 Autoantibody Radical Ligand by TRIS(77-86-1) Buffer Solution
    Tris(77-86-1) buffer was used for the determination of protein quantification
    Total selenium and inorganic selenium were extracted by microwave digestion and tris(77-86-1) - hydrochloric acid buffer solution
    Tris(cas 77-86-1)-hydroxymethylaminomethane hydrochloride as raw material
    Tris(77-86-1) is used in the determination of salinomycin in feed
    50 mM TRIS(77-86-1)-hydrochloric acid electrophoresis buffer
    TRIS(77-86-1) as a heat stabilizer for polyvinyl chloride
    Determination of serum glutamate dehydrogenase by using TRIS(cas 77-86-1) - hydrochloric acid as buffer
    TRIS(cas 77-86-1)-HCl buffer solution instead of distilled water
    Lauric acid and Tris(cas 77-86-1) as buffer solution
    TRIS(77-86-1) solution was used for capillary electrophoresis test
    The sample was extracted with a mixture of TRIS(cas 77-86-1) and calcium chloride
    TRIS(77-86-1), potassium carbonate and hydrochloric acid as raw materials
    TRIS(cas 77-86-1) was used for capillary gel electrophoresis to detect nucleic acid sensitivity
    The TRIS(cas 77-86-1) buffer solution was used to determine the quercetin content in the ethanol extract of Podocarpus
    The background electrolyte containing 0.02 mol.L-1 TRIS(cas 77-86-1)
    TRIS(cas 77-86-1) and sodium edetate as adjuvants
    NPG, TRIS(77-86-1) and its binary system by solid - solid phase change DSC measurement


Chemical Name:TRIS
CAS No.:77-86-1
Appearance: White crystal powder
Molecular Formula:C4H11NO3
Molecular Weight:121.14
Packing: 25kg fiber drum

TRIS(77-86-1) Chemical Properties:
White crystal or powder. Melting point 171-172℃, boiling point 219-220℃/1.3kPa, soluble in ethanol and water, slightly soluble in ethyl acetate, benzene, insoluble in ether, carbon tetrachloride,copper,aluminum corrosion effects,irritant.

TRIS(77-86-1) is prepared industrially by the exhaustive condensation of nitromethane with formaldehyde under basic conditions (i.e. repeated nitroaldol reactions) to produce the intermediate (HOCH2)3CNO2, which is subsequently hydrogenated to give the final product.

Buffering features:
TRIS(77-86-1) has a pKa of 8.07 at 25 °C, which implies that the buffer has an effective pH range between 7.5 and 9.0.

Buffer details:
The pKa declines approximately 0.03 units per degree Celsius rise in temperature.
Silver-containing single-junction pH electrodes (e.g., silver chloride electrode) are incompatible with TRIS(77-86-1) (Ag-TRIS(77-86-1) precipitation clogs the junction). Double-junction electrodes are resistant to this problem, and non-silver containing electrodes are immune.
Making buffer solutions by neutralizing TRIS(77-86-1) HCl requires attention to the attendant changes in ionic strength.

Buffer inhibition:
TRIS(77-86-1) inhibits a number of enzymes, and therefore it should be used with care when studying proteins.

TRIS(77-86-1) Uses of Biology:
TRIS(77-86-1) base, also known as THAM, is widely used as a biological buffer or as a component of buffer formulations such as TAE (sc-296645) and TBE (sc-296650) buffers. TRIS(77-86-1) has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). TRIS(77-86-1) has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.

TRIS(77-86-1)(hydroxymethyl)aminomethane is used as buffers in biochemistry and molecular biology laboratories. It is used as a primary standard to standardize acid solutions for chemical analysis. It finds application in cell membranes to increase its permeability. As an alternative to sodium bicarbonate, it used in the treatment of metabolic acidosis.

The useful buffer range for TRIS(77-86-1) (7-9) coincides with the physiological pH typical of most living organisms. This, and its low cost, make TRIS(77-86-1) one of the most common buffers in the biology/biochemistry laboratory. TRIS(77-86-1) is also used as a primary standard to standardize acid solutions for chemical analysis.

TRIS(77-86-1) is used to increase membrane permeability of cell membranes.
1, TRIS(77-86-1) buffer, only nucleic acids and proteins are widely used as solvents.
2, TRIS(77-86-1) is used for protein crystal growth under different pH conditions.
3, TRIS(77-86-1) buffer having a low ionic strength can be used nematode (C. elegans) is formed lamin (lamin) intermediate fibers.
4, TRIS(77-86-1) is a major component of protein electrophoresis buffer.
5, TRIS(77-86-1) for the preparation of a surfactant, a vulcanization accelerator, and some drug intermediates.
6, TRIS(77-86-1) titrated also used as standard.

TRIS(77-86-1) (usually known as THAM in this context) is used as alternative to sodium bicarbonate in the treatment of metabolic acidosis.

 77-86-1 77-86-1 77-86-1

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