HEPES(cas 7365-45-9) buffer is necessary for the cell culture process
    RNA extraction solution: HEPES(cas 7365-45-9) and sodium chloride
    DMSO /H2O (VV = 31) HEPES(cas 7365-45-9) buffer system
    Hank balanced salt solution added with HEPES(cas 7365-45-9)
    HEPES(cas 7365-45-9) is used for the separation and primary culture of macrophages from the rhubarb
    The vesicle probe was blue and had no fluorescence in HEPES(cas 7365-45-9) buffer
    Do experiments with HEPES(cas 7365-45-9)
    Purchased HEPES(cas 7365-45-9) from XZL Bio-Technology Co., Ltd.
    HEPES(7365-45-9) medium for the regulation of T cells cultured and induced differentiation
    DMF(N, N-dimethylformamide) -HEPES(cas 7365-45-9) buffer
    HEPES(7365-45-9) solution as buffer to detect polyhexamethylene guanidine content
    0.01 mol /L Hepes(7365-45-9)
    Red blood cells are suspended in HEPES(7365-45-9) and plasma
    HEPES(cas 7365-45-9) inhibits extracellular acidification to significantly inhibit cell killing
    Cr (Ⅵ) - GSH intermediate complex was formed in HEPES(7365-45-9) buffer solution
    DMF-HEPES(cas 7365-45-9) buffer
    Physiological buffer: HEPES(7365-45-9)
    Hydroxyethyl piperazineethanesulfonic acid(cas 7365-45-9)
    The cells were washed with and suspend in HEPES(7365-45-9) buffer
    Sensitivity of Polymers to Fe ~ (3+) and Inorganic Phosphate (Pi) in HEPES(7365-45-9) Buffer Solution(pH 7.0)


Chemical Name:HEPES
CAS #:7365-45-9
Appearance: White crystal powder
Molecular Formula:C8H18N2O4S
Molecular Weight:238.31
Packing: 25kg plastic drum

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES(7365-45-9)) is a zwitterionic organic buffer used in cellular and molecular biology applications.
HEPES(7365-45-9) is frequently employed in isoelectric focusing and cell culture applications. This buffer has a useful pH range of 6.8 to 8.2.
Product Name: HEPES
Product Grade: Molecular Biology Grade
Alternative Names: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid; 4-(2-Hydroxyethyl)piperazine-1-ethane-sulfonic acid; HEPES Free Acid; HEPES Buffer; C8H18N2O4S
Storage: Room Temperature, Protected From Moisture

HEPES(7365-45-9)  Specifications:
Appearance: White Crystalline Powder
Purity: is ≥ 99.6%
Heavy Metals: 10 ppm max;
A260 nm: 0.050 max
A280 nm: 0.050 max
Solubility: To Pass Test
Ignition Residue: 0.05% max
Sulfate: 0.05% max

HEPES(7365-45-9)  Properties:
Physical State: Solid
CAS Number: 7365-45-9
Chemical Formula: C8H18N2O4S
Molecular Weight: 283.30

HEPES(7365-45-9) (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent; one of the twenty Good's buffers. HEPES(7365-45-9) is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES(7365-45-9) is like water in that its dissociation decreases as the temperature decreases. This makes HEPES(7365-45-9) a more effective buffering agent for maintaining enzyme structure and function at low temperatures. Lepe-Zuniga et al. reported a phototoxicity of HEPES(7365-45-9) when exposed to ambient light by the production of hydrogen peroxide, which is not a problem in bicarbonate-based cell culture buffers. It is therefore strongly advised to keep HEPES(7365-45-9)-containing solutions in darkness as much as possible.

HEPES(7365-45-9) has the following characteristics:
pKa1 (25 °C) = 3
pKa2 (25 °C) = 7.5
Useful pH range = 2.5 to 3.5 or 6.8 to 8.2

 7365-45-9 7365-45-9 7365-45-9

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